Journal: RSC Advances

Article Title: One-pot aqueous synthesis of highly strained CdTe/CdS/ZnS nanocrystals and their interactions with cells

doi: 10.1039/c4ra13386b

Figure Lengend Snippet: Fig. 4 Viability of A549 adenocarcinoma lung cancer cells treated with CS 550 (CdTe/CdS, lemission ¼ 550 nm), CS 659 (CdTe/CdS, lemission ¼ 659 nm) and its corresponding CSS 0.5 ML (CdTe/CdS/ZnS, 0.5 ML ZnS) and CSS 1 ML (CdTe/CdS/ZnS, 1 ML ZnS) with a concen- tration range 0.002–600 mg ml1. CellTiter-Blue assay was performed with the cells incubated with with NCs for 2 h (a) and 24 h (b). Half- maximal inhibitory concentration (IC50) values were calculated by fitted dose–response curves (presented data as mean standard deviation of 4 replicates).

Article Snippet: For the cytotoxicity studies, human lung adenocarcinoma A549 cells (ACC107) purchased from DSMZ (German Collection of Microorganism and Cell Cultures) were used with a passage number of less than 20.

Techniques: CtB Assay, Incubation, Concentration Assay, Standard Deviation

Journal: RSC Advances

Article Title: One-pot aqueous synthesis of highly strained CdTe/CdS/ZnS nanocrystals and their interactions with cells

doi: 10.1039/c4ra13386b

Figure Lengend Snippet: Fig. 5 Fluorescence microscope images of A549 adenocarninoma lung cancer cells treated with S15 aptamer–NCs conjugates (a), Oligo– NCs (c), and bare NCs (d) for 2 h. Images of cells treated with S15–NCs after 24 h of internalization (b). Cell nuclei were counterstained with DAPI. Scale bar 50 mm.

Article Snippet: For the cytotoxicity studies, human lung adenocarcinoma A549 cells (ACC107) purchased from DSMZ (German Collection of Microorganism and Cell Cultures) were used with a passage number of less than 20.

Techniques: Fluorescence, Microscopy

Journal: Microorganisms

Article Title: Carboxy-Terminal Processing Protease Controls Production of Outer Membrane Vesicles and Biofilm in Acinetobacter baumannii

doi: 10.3390/microorganisms9061336

Figure Lengend Snippet: ctp mutation led to the hypervesiculation phenotype. ( A ) TEM analysis of membrane vesicles obtained from ATCC 17978, MR14 and MR14C. OMVs and OMTs are indicated by black and white arrows, respectively. ( B ) Left graph: OMV quantification by using the BCA method; right image: results of the SDS-PAGE analysis of OMVs obtained from ATCC 17978 and MR14; and bottom image: results of the Western blotting analysis using an antibody against 38-kDa OmpA. ( C ) Quantification of eDNA associated with OMVs of ATCC 17978 and MR14. ( D ) and ( E ) Histograms of size and particle distribution obtained using nanoparticle tracking analysis of OMVs isolated from ATCC 17978 and MR14, respectively. Graphs represent the mean ± SD of three independent replicates. *** p < 0.001 and **** p < 0.0001.

Article Snippet: The MR14 OMVs were more cytotoxic toward A549 cells than ATCC 17978 OMVs.

Techniques: Mutagenesis, SDS Page, Western Blot, Isolation

Journal: Microorganisms

Article Title: Carboxy-Terminal Processing Protease Controls Production of Outer Membrane Vesicles and Biofilm in Acinetobacter baumannii

doi: 10.3390/microorganisms9061336

Figure Lengend Snippet: ctp mutation increases OMV-mediated host cell cytotoxicity. The host cell cytotoxicity was measured by treating A549 cells with different concentrations of OMVs isolated from ATCC 17978 and MR14 for 24 h and then performing an MTT assay. Data are presented as mean ± SD of three independent experiments + p < 0.05 compared with untreated control. ** p < 0.01 for comparison of cytotoxicity at the same concentration of OMVs between ATCC 17978 and MR14.

Article Snippet: The MR14 OMVs were more cytotoxic toward A549 cells than ATCC 17978 OMVs.

Techniques: Mutagenesis, Isolation, MTT Assay, Concentration Assay